LOCUS       40924_22809        7099 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Moss transformation vector pGUSmutNPTII DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7099)
  AUTHORS   Koshimizu S, Aono N, Sasaki-Sekimoto Y, Shigenobu S, Shimojima M, 
            Ohta H, Kofuji R, Tamada Y, Murata T, Hasebe M.
  TITLE     Physcomitrella MADS-box genes regulate external water conduction and
            sperm movement necessary for fertilization
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7099)
  AUTHORS   Kofuji R, Koshimizu S, Hasebe M.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JUN-2017) Contact:Shizuka Koshimizu National Institute
            for Basic Biology, Division of Evolutionary Biology; Nishigonaka 38,
            Myodaiji, Okazaki, Aichi 444-8585, Japan
REFERENCE   3  (bases 1 to 7099)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7099)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (23-JUN-2017) Contact:Shizuka Koshimizu National Institute for Basic
            Biology, Division of Evolutionary Biology; Nishigonaka 38, Myodaiji,
            Okazaki, Aichi 444-8585, Japan"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7099
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..37
                     /label=pBluescriptII KS
                     /note="pBluescriptII KS"
     primer_bind     18..34
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             43..1851
                     /codon_start=1
                     /label=GUS
                     /note="beta-glucuronidase"
                     /translation="LLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRAI
                     AVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQEV
                     MEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSYFHD
                     FFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRDADQQ
                     VVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAVKGEQF
                     LINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAEEMLDW
                     ADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARD
                     KNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCDAHTDTISD
                     LFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMY
                     TDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKKGIFTRDRK
                     PKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     terminator      1925..2177
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        2262..2607
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             2637..3428
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPXELFAXLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    3501..3677
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     primer_bind     complement(4206..4222)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(4255..4273)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4283..4299)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      4441..4896
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        4922..5026
                     /label=AmpR promoter
     CDS             5027..5884
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6058..6646
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    6934..6955
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        6970..7000
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7008..7024
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7032..7048
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        7069..7087
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ggtaccgggc cccccctcga ggtcgacggt atcgataagc ttttgttacg tcctgtagaa
       61 accccaaccc gtgaaatcaa aaaactcgac ggcctgtggg cattcagtct ggatcgcgaa
      121 aactgtggaa ttgatcagcg ttggtgggaa agcgcgttac aagaaagccg ggcaattgct
      181 gtgccaggca gttttaacga tcagttcgcc gatgcagata ttcgtaatta tgcgggcaac
      241 gtctggtatc agcgcgaagt ctttataccg aaaggttggg caggccagcg tatcgtgctg
      301 cgtttcgatg cggtcactca ttacggcaaa gtgtgggtca ataatcagga agtgatggag
      361 catcagggcg gctatacgcc atttgaagcc gatgtcacgc cgtatgttat tgccgggaaa
      421 agtgtacgta tcaccgtttg tgtgaacaac gaactgaact ggcagactat cccgccggga
      481 atggtgatta ccgacgaaaa cggcaagaaa aagcagtctt acttccatga tttctttaac
      541 tatgccggaa tccatcgcag cgtaatgctc tacaccacgc cgaacacctg ggtggacgat
      601 atcaccgtgg tgacgcatgt cgcgcaagac tgtaaccacg cgtctgttga ctggcaggtg
      661 gtggccaatg gtgatgtcag cgttgaactg cgtgatgcgg atcaacaggt ggttgcaact
      721 ggacaaggca ctagcgggac tttgcaagtg gtgaatccgc acctctggca accgggtgaa
      781 ggttatctct atgaactgtg cgtcacagcc aaaagccaga cagagtgtga tatctacccg
      841 cttcgcgtcg gcatccggtc agtggcagtg aagggcgaac agttcctgat taaccacaaa
      901 ccgttctact ttactggctt tggtcgtcat gaagatgcgg acttgcgtgg caaaggattc
      961 gataacgtgc tgatggtgca cgaccacgca ttaatggact ggattggggc caactcctac
     1021 cgtacctcgc attaccctta cgctgaagag atgctcgact gggcagatga acatggcatc
     1081 gtggtgattg atgaaactgc tgctgtcggc tttaacctct ctttaggcat tggtttcgaa
     1141 gcgggcaaca agccgaaaga actgtacagc gaagaggcag tcaacgggga aactcagcaa
     1201 gcgcacttac aggcgattaa agagctgata gcgcgtgaca aaaaccaccc aagcgtggtg
     1261 atgtggagta ttgccaacga accggatacc cgtccgcaag gtgcacggga atatttcgcg
     1321 ccactggcgg aagcaacgcg taaactcgac ccgacgcgtc cgatcacctg cgtcaatgta
     1381 atgttctgcg acgctcacac cgataccatc agcgatctct ttgatgtgct gtgcctgaac
     1441 cgttattacg gatggtatgt ccaaagcggc gatttggaaa cggcagagaa ggtactggaa
     1501 aaagaacttc tggcctggca ggagaaactg catcagccga ttatcatcac cgaatacggc
     1561 gtggatacgt tagccgggct gcactcaatg tacaccgaca tgtggagtga agagtatcag
     1621 tgtgcatggc tggatatgta tcaccgcgtc tttgatcgcg tcagcgccgt cgtcggtgaa
     1681 caggtatgga atttcgccga ttttgcgacc tcgcaaggca tattgcgcgt tggcggtaac
     1741 aagaaaggga tcttcactcg cgaccgcaaa ccgaagtcgg cggcttttct gctgcaaaaa
     1801 cgctggactg gcatgaactt cggtgaaaaa ccgcagcagg gaggcaaaca atgaatcaac
     1861 aactctcctg gcgcaccatc gtcggctaca gcctcgggaa ttgctaccga gctcgaattt
     1921 ccccgatcgt tcaaacattt ggcaataaag tttcttaaga ttgaatcctg ttgccggtct
     1981 tgcgatgatt atcatataat ttctgttgaa ttacgttaag catgtaataa ttaacatgta
     2041 atgcatgacg ttatttatga gatgggtttt tatgattaga gtcccgcaat tatacattta
     2101 atacgcgata gaaaacaaaa tatagcgcgc aaactaggat aaattatcgc gcgcggtgtc
     2161 atctatgtta ctagatcggg aattctgcag cccatcgaat tcctgcagcc cccctactcc
     2221 aaaaatgtca aagatacagt ctcagaagac caaagggcta ttgagacttt tcaacaaagg
     2281 gtaatttcgg gaaacctcct cggattccat tgcccagcta tctgtcactt catcgaaagg
     2341 acagtagaaa aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggctatc
     2401 attcaagatg cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc
     2461 gtggaaaaag aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgacatctcc
     2521 actgacgtaa gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa
     2581 ggaagttcat ttcatttgga gaggacagcc caagcatgga gaaccgacct gcaggcatga
     2641 ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct
     2701 atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc
     2761 aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactccaag
     2821 acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg
     2881 acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc
     2941 tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc
     3001 ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg
     3061 agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc
     3121 atcaggggct cgcgccakcc gaactgttcg ccangctcaa ggcgcggatg cccgacggcg
     3181 aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc
     3241 gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag
     3301 cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg
     3361 tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg
     3421 agttcttctg agcgggactc tggggttcgg acggtacgct gaaatcacca gtctctctct
     3481 acaaatctat ctctctctat tttctccata aataatgtgt gagtagtttc ccgataaggg
     3541 aaattagggt tcttataggg tttcgctcat gtgttgagca tataagaaac ccttagtatg
     3601 tatttgtatt tgtaaaatac ttctatcaat aaaatttcta attcctaaaa ccaaaatcca
     3661 gtactaaaat ccagatctcc taaagtccct atagatcttt gtcgtgaata taaaccagac
     3721 acgagacgac taaacctgga gcccagacgc cgttcgaagc tagaagtacc gcttaggcag
     3781 gaggccgtta gggaaaagat gctaaggcag ggttggttac gttgactccc ccgtaggttt
     3841 ggtttaaata tgatgaagtg gacggaagga aggaggaaga caaggaagga taaggttgca
     3901 ggccctgtgc aaggtaagaa gatggaaatt tgatagaggt acgctactat acttatacta
     3961 tacgctaagg gaatgcttgt atttataccc tataccccct aataacccct tatcaattta
     4021 agaaataatc cgcataagcc cccgcttaaa aattggtatc agagccatga ataggtctat
     4081 gaccaaaact caagaggata aaacctcacc aaaatacgaa agagttctta actctaaaga
     4141 taaaagatct ttcaagatca aaactagttc cctcacaccg gtgacgggga tcgcatgcga
     4201 tgggggatcc actagttcta gagcggccgc caccgcggtg gagctccaat tcgccctata
     4261 gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac tgggaaaacc
     4321 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata
     4381 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggg
     4441 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     4501 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     4561 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     4621 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
     4681 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     4741 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
     4801 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     4861 acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaatgt
     4921 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     4981 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     5041 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     5101 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     5161 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     5221 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     5281 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     5341 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     5401 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     5461 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     5521 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     5581 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     5641 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     5701 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     5761 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     5821 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     5881 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     5941 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     6001 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     6061 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     6121 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     6181 cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa
     6241 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     6301 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     6361 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     6421 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     6481 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     6541 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     6601 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     6661 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     6721 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     6781 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
     6841 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
     6901 cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc
     6961 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata
     7021 acaatttcac acaggaaaca gctatgacca tgattacgcc aagcgcgcaa ttaaccctca
     7081 ctaaagggaa caaaagctg
//
